Batch Kinetics and Modeling of Alkaline Protease Production by Isolated Bacillus sp. (RESEARCH NOTE)
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Abstract:
The aim of this study was the use of fish waste hydrolysate (FWH) as a substrate for alkaline protease production using isolated Bacillus sp. in a batch system. Then the fermentation kinetics of enzyme production was studied. The results show that with the addition of FWH to the fermentation medium with a final concentration of 4% (optimal concentration), alkaline protease value reached a maximum value (89 U/ml), which is 63% higher than that of the control medium. Also fermentation kinetics of alkaline protease by isolated Bacillus sp. was studied. Several kinetic models were evaluated; the combination of the Moser and Boulton kinetic model gave the best prediction. This nonlinear mathematical model performed satisfactory on biomass, substrate, and enzyme predictions.
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Journal title
volume 28 issue 6
pages 841- 847
publication date 2015-06-01
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